{"created":"2023-06-20T13:10:20.210620+00:00","id":1153,"links":{},"metadata":{"_buckets":{"deposit":"1eec9129-dded-4232-a79f-c580fe2485b6"},"_deposit":{"created_by":3,"id":"1153","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1153"},"status":"published"},"_oai":{"id":"oai:kobe-tokiwa.repo.nii.ac.jp:00001153","sets":["6:77"]},"author_link":["7232","7231","7234","7233","7097","7095","6045","7096"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"14","bibliographicPageEnd":"113","bibliographicPageStart":"104","bibliographic_titles":[{"bibliographic_title":"神戸常盤大学紀要"},{"bibliographic_title":"Bulletin of Kobe Tokiwa University","bibliographic_titleLang":"en"}]}]},"item_10002_description_25":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"We investigated a reagent kit for performing the indirect immunofluorescence assay (IF) on a sample after immunoabsorption of anti-DFS70 antibody with DFS70 antigen. The positivity rate of antinuclear antibody (ANA) was compared between the conventional IF and this method in 247 healthy individuals(HI), and the DFS pattern by the conventional IF and this method was compared with the presence of anti-DFS70 antibody by enzyme-linked immunosorbent assay ( ELISA). Furthermore, the ANA positivity rate of HI after immunoabsorption with a nti-DFS70 antibody was examined. The ANA positivity rate by the conventional IF and this method were 15.0% and 19.8%, respectively. Forty-nine cases were positive without immunoabsorption, and 38 cases were positive after immunoabsorption, with the number of ANA positives decreasing by 11 cases. The frequency of the DFS pattern was 9.7% and 12.6% in the conventional IF and this method, respectively. However, there were cases that showed DFS pattern after immunoabsorption. Although this method is useful as an IF method that suppresses the detection of DFS pattern, immunoabsorption with t he DFS70 a ntigen may not be sufficient."}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"抗DFS70抗体をDFS70抗原で免疫吸収処理後に間接蛍光抗体法(IF法)を実施する検査を検討した。健常人247名を対象とし、抗核抗体陽性率を従来のIF法と本法を比較し、従来のIF法と本法によるDFS型判定とELISA法による抗DFS70抗体の有無を比較する。さらに、抗DFS70抗体を吸収処理後の健常人の抗核抗体陽性率を調べた。抗核抗体の陽性率は従来のIF法、本法で、それぞれ15.0%、19.8%であった。本法での免疫吸収未処理の陽性例は49例、免疫吸収処理後の陽性例は38例と、抗核抗体陽性数が11例減少した。DFS型出現頻度は従来のIF法、本法で、それぞれ9.7%、12.6%であった。しかし、免疫吸収処理後でDFS型を示した例があった。本法は、DFS型の検出を抑制する点でIF法の検査法として有用であるが、DFS70抗原での吸収処理は不十分であるかもしれない。"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.20608/00001143","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神戸常盤大学・神戸常盤大学短期大学部"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18845487","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"林, 伸英"},{"creatorName":"ハヤシ, ノブヒデ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"今西, 麻樹子"},{"creatorName":"イマニシ, アキコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"生戸, 健一"},{"creatorName":"ウト, ケンイチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"三枝, 淳"},{"creatorName":"サエグサ, ジュン","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"HAYASHI, Nobuhide","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IMANISHI, Akiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"UTO, Kenichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAEGUSA, Jun","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-03-29"}],"displaytype":"detail","filename":"Bulletin14_15.pdf","filesize":[{"value":"5.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Bulletin14_15.pdf","url":"https://kobe-tokiwa.repo.nii.ac.jp/record/1153/files/Bulletin14_15.pdf"},"version_id":"0e23707a-b4af-42ae-abad-8abdc7bac193"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"抗核抗体"},{"subitem_subject":"間接蛍光抗体法"},{"subitem_subject":"抗DFS70抗体"},{"subitem_subject":"吸収処理"},{"subitem_subject":"健常人"},{"subitem_subject":"antinuclear antibodies"},{"subitem_subject":"immunofluorescence assay"},{"subitem_subject":"DFS70"},{"subitem_subject":"immunoabsorption"},{"subitem_subject":"healthy individuals"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"免疫吸収処理を用いて抗DFS70抗体を除外する間接蛍光抗体法による抗核抗体検査の評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"免疫吸収処理を用いて抗DFS70抗体を除外する間接蛍光抗体法による抗核抗体検査の評価"},{"subitem_title":"The Evaluation of immunoadsorption method for anti-DFS70 antibodies by indirect immunofluorescence assay of antinuclear antibody","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"3","path":["77"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-03-31"},"publish_date":"2021-03-31","publish_status":"0","recid":"1153","relation_version_is_last":true,"title":["免疫吸収処理を用いて抗DFS70抗体を除外する間接蛍光抗体法による抗核抗体検査の評価"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T13:15:32.154867+00:00"}