{"created":"2024-03-25T05:06:54.534545+00:00","id":2000040,"links":{},"metadata":{"_buckets":{"deposit":"77e247ec-596d-416e-9f82-6c97cb99ff66"},"_deposit":{"created_by":13,"id":"2000040","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"2000040"},"status":"published"},"_oai":{"id":"oai:kobe-tokiwa.repo.nii.ac.jp:02000040","sets":["6:1695863573345"]},"author_link":[],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"17","bibliographicPageEnd":"53","bibliographicPageStart":"46","bibliographic_titles":[{"bibliographic_title":"神戸常盤大学紀要","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of Kobe Tokiwa University","bibliographic_titleLang":"en"}]}]},"item_10002_description_25":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Although the inhibitory or proliferative role of estrogen in the colorectal mucosa has been previously studied, the results remain controversial. To help resolve these controversies, we assessed cell death and proliferation in the colorectal mucosa of ovariectomized mice to determine the effects of estrogen. In addition, the effect of magnesium (Mg) administration was evaluated, as Mg is associated with osteoporosis prevention and alleviation of colorectal cancer risks. Proteins expression was analyzed in three groups of mouse colorectal tissue sections (OVX: ovariectomized, OVX + Mg: ovariectomized + Mg administration, control: sham operation) by immunostaining using antibodies specific for caspase 3, beclin 1, cyclin D1, and Ki-67. Caspase 3 expression was significantly higher in the OVX group than in control mice. No significant differences in beclin 1, cyclin D1, and Ki-67 expression were observed between OVX and control mice. Comparing the OVX + Mg colorectal tissue sections with the controls, caspase 3 expression was significantly higher in the OVX + Mg group, whereas beclin 1 were significantly lower in the OVX + Mg group. These results suggest that apoptosis could be accelerated in the colorectum of ovariectomized\nmice. Furthermore, Mg administration might promote apoptosis a nd inhibit autophagy."}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"卵巣摘出マウスの大腸粘膜における細胞増殖および細胞死関連タンパクの発現を解析し、エストロゲンの大腸粘膜への作用について検討した。3群(OVX：卵巣摘出、OVX＋Mg：卵巣摘出＋マグネシウム投与、コントロール：偽手術)のマウス結腸・直腸切片を対象とし、caspase 3、beclin 1、cyclin D1、Ki-67 の各抗体を用いて免疫組織化学染色を行った。OVXとコントロールを比較すると、beclin 1、cyclin D1、Ki-67の発現に有意差は認められなかったが、caspase 3の発現はOVXで有意に高くなった。OVX＋Mg とコントロールを比較すると、caspase 3の発現はOVX＋Mgで有意に高く、beclin 1はOVX＋Mgで有意に低かった。以上から、卵巣摘出マウス大腸ではアポトーシスが促進し、さらにMg投与はアポトーシスを促進させオートファジーを抑制する可能性が示唆された。"}]},"item_10002_full_name_3":{"attribute_name":"著者（英）","attribute_value_mlt":[{"names":[{"name":"SHINTANI, Michiko","nameLang":"en"}]},{"names":[{"name":"IMANISHI, Akiko","nameLang":"en"}]},{"names":[{"name":"YOSHIKAWA, Yutaka","nameLang":"en"}]},{"names":[{"name":"KAMOSHIDA, Shingo","nameLang":"en"}]}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神戸常盤大学・神戸常盤大学短期大学部"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18845487","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"新谷, 路子","creatorNameLang":"ja"},{"creatorName":"シンタニ, ミチコ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"今西, 麻樹子","creatorNameLang":"ja"},{"creatorName":"イマニシ, アキコ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"吉川, 豊","creatorNameLang":"ja"},{"creatorName":"ヨシカワ, ユタカ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"鴨志田, 伸吾","creatorNameLang":"ja"},{"creatorName":"カモシダ, シンゴ","creatorNameLang":"ja-Kana"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2024-03-31"}],"displaytype":"detail","filename":"Bulletin17_09.pdf","format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Bulletin17_09.pdf","url":"https://kobe-tokiwa.repo.nii.ac.jp/record/2000040/files/Bulletin17_09.pdf"},"version_id":"874e7fdd-bce4-463a-b6df-7e68ac2b2540"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"大腸粘膜"},{"subitem_subject":"細胞増殖"},{"subitem_subject":"アポトーシス"},{"subitem_subject":"エストロゲン"},{"subitem_subject":"colorectal mucosa"},{"subitem_subject":"cell proliferation"},{"subitem_subject":"apoptosis"},{"subitem_subject":"estrogen"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"卵巣摘出マウス大腸における細胞増殖・細胞死関連蛋白の発現","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"卵巣摘出マウス大腸における細胞増殖・細胞死関連蛋白の発現","subitem_title_language":"ja"},{"subitem_title":"Expression of proteins related to cell death and proliferation in the colorectal mucosa of ovariectomized mice","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"13","path":["1695863573345"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-03-31"},"publish_date":"2024-03-31","publish_status":"0","recid":"2000040","relation_version_is_last":true,"title":["卵巣摘出マウス大腸における細胞増殖・細胞死関連蛋白の発現"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2024-04-08T05:47:42.249823+00:00"}