{"created":"2023-06-20T13:09:46.178611+00:00","id":399,"links":{},"metadata":{"_buckets":{"deposit":"ba0196de-518c-4806-a89e-a79874d7da06"},"_deposit":{"created_by":3,"id":"399","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"399"},"status":"published"},"_oai":{"id":"oai:kobe-tokiwa.repo.nii.ac.jp:00000399","sets":["6:10"]},"author_link":["1917","2032","4693","1624","4695","4697","4698","4694","4696","1919","1915","2033","4692","4699"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"50","bibliographicPageStart":"43","bibliographic_titles":[{"bibliographic_title":"神戸常盤大学紀要"},{"bibliographic_title":"Bulletin of Kobe Tokiwa University","bibliographic_titleLang":"en"}]}]},"item_10002_description_25":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Hemin, heme-derived molecule, can induce reactive oxygen species (ROS) production and cell death. However, effects of hemin on monocyte/macrophage remain unclear. The purpose of this study was to understand the mechanisms of ROS production and cell death induced by hemin in monocyte/macrophage. Human monocytic THP-1 cells were treated with hemin and cell death and ROS production were measured by flow cytometry. Hemin dose-dependently induced cell death and ROS production. The anti-oxidant N-acetyl cysteine (NAC) suppressed both hemin-induced ROS production and cell death. The mitochondria-selective anti-oxidant MitoTEMPO suppressed hemin-induced cell but not ROS production. Among the NADPH oxidase inhibitors, diphenyleneiodonium chloride (DPI), apocynin, and VAS2870, only DPI suppressed hemin-induced ROS production and cell death. These results indicate that the NADPH oxidase pathway is not the main pathway for hemin-induced ROS production, and that ROS-producing reactions in mitochondria are important for hemin induced cell death."}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヘム誘導体がマクロファージの細胞死を誘導する機構についてはまだ不明な点が多い。本研究の目的は、ヘム誘導体へミンが単球/ マクロファージのROS 産生と細胞死を誘導する機構の解明である。ヒト単球系培養細胞THP-1を用いて、へミンによるROS 産生と細胞死をフローサイトメトリー（FCM）等で測定した。へミンは濃度依存的に細胞死とROS 産生を誘導した。抗酸化剤NAC はROS 産生と細胞死を抑制し、ミトコンドリア選択的抗酸化剤MitoTEMPO は細胞死のみを抑制した。NADPH oxidase（Nox）阻害剤では、DPIはROS 産生と細胞死を抑制したが、アポサイニンとNox 特異的阻害剤VAS2870はROS 産生も細胞死も抑制しなかった。以上の結果から、１）へミンによるROS 産生には、複数経路が関与していること、２）へミンによる細胞死誘導にはミトコンドリアでのROS 作用が重要であること、を示している。"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.20608/00000390","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神戸常盤大学・神戸常盤大学短期大学部"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"21884781","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"井本, しおん"},{"creatorName":"イモト, シオン","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"澤村, 暢"},{"creatorName":"サワムラ, トオル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"溝越, 祐志"},{"creatorName":"ミゾコシ, ユウジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"渋谷, 雪子"},{"creatorName":"シブヤ, ユキコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"澤田, 浩秀"},{"creatorName":"サワダ, ヒロヒデ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"西郷, 勝康"},{"creatorName":"サイゴウ, カツヤス","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"河野, 麻理"},{"creatorName":"コウノ, マリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IMOTO, Shion","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAWAMURA, Tohru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MIZOKOSHI, Yuji","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHIBUYA, Yukiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAWADA, Hirohide","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAIGO, Katsuyasu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KONO, Mari","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-07-25"}],"displaytype":"detail","filename":"Bulletin10_08.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Bulletin10_08.pdf","url":"https://kobe-tokiwa.repo.nii.ac.jp/record/399/files/Bulletin10_08.pdf"},"version_id":"ec17fe88-a50b-4b91-a130-661cf7e7215f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"へミン"},{"subitem_subject":"単球／マクロファージ"},{"subitem_subject":"THP-1"},{"subitem_subject":"細胞死"},{"subitem_subject":"ROS"},{"subitem_subject":"hemin"},{"subitem_subject":"monocyte/macrophage"},{"subitem_subject":"THP-1"},{"subitem_subject":"cell death"},{"subitem_subject":"ROS"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"ヘム誘導体が単球/ マクロファージのROS 産生と細胞死を誘導する機構の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヘム誘導体が単球/ マクロファージのROS 産生と細胞死を誘導する機構の検討"},{"subitem_title":"Mechanism of hemin-induced ROS production and cell death in monocyte/macrophage","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"3","path":["10"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-03-31"},"publish_date":"2017-03-31","publish_status":"0","recid":"399","relation_version_is_last":true,"title":["ヘム誘導体が単球/ マクロファージのROS 産生と細胞死を誘導する機構の検討"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T13:17:08.146698+00:00"}