@article{oai:kobe-tokiwa.repo.nii.ac.jp:00000440,
 author = {北野, 悦子 and 畑中, 道代 and 北村, 肇 and KITANO, Etsuko and HATANAKA, Michiyo and Kitamura, Hajime},
 issue = {1},
 journal = {神戸常盤大学紀要, Bulletin of Kobe Tokiwa University},
 month = {Nov},
 note = {Serum complement activities of human and several experimental animals are usually estimated by the hemolytic assay using sensitized sheep erythrocytes and/or unsensitized rabbit erythrocytes. However, it is well known that these erythrocytes are hardly lysed by incubation with mouse serum and that mouse serum complement activity cannot be measured easily by hemolytic or any other assay.  The present study deals with a new hemolytic assay for complement activities of mouse serum, in which the activity is estimated from the degree of hemolysis of unsensitized sheep erythrocytes after incubation of serum in the presence of zymosan.  It was confirmed that serum complement activation is responsible for the hemolysis, since the hemolysis was not observed when erythrocytes were incubated  i） in buffer containing EDTA, ii） by heatinactivated serum, iii） by C5-deficient mouse serum or iv） in the absence of zymosan.  The mechanism of hemolysis is  so-called the reactive lysis（ deviated lysis, bystander lysis）, in which erythrocytes are lysed when serum complement activation proceeds not on the erythrocyte membrane but in the fluid phase close to the erythrocytes. Since this assay is simply and easily handled, and does not require any special reagent or treatment of serum and cells, the method may contribute widely to experimental biology.},
 pages = {1--8},
 title = {マウス血清の補体活性測定法の確立－ザイモザン活性化によるreactive lysisを利用した方法の検討－},
 year = {2009},
 yomi = {キタノ, エツコ and ハタナカ, ミチヨ and キタムラ, ハジメ}
}