{"created":"2023-06-20T13:10:08.835987+00:00","id":973,"links":{},"metadata":{"_buckets":{"deposit":"118125b8-f1bd-446b-b63b-7b600c7149ac"},"_deposit":{"created_by":3,"id":"973","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"973"},"status":"published"},"_oai":{"id":"oai:kobe-tokiwa.repo.nii.ac.jp:00000973","sets":["6:71"]},"author_link":["6082","6084","7740","7738","6083","7739"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"106","bibliographicPageStart":"97","bibliographic_titles":[{"bibliographic_title":"神戸常盤大学紀要"},{"bibliographic_title":"Bulletin of Kobe Tokiwa University","bibliographic_titleLang":"en"}]}]},"item_10002_description_25":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"Presepsin, produced by CD14 degradation in monocytes, is one of the biomarker proteins for sepsis. Moreover, healthy people also have presepsin ; however, no studies have elucidated the mechanism of presepsin production in healthy bodies. To clarify the mechanism of presepsin production in healthy individuals, we studied whether HL-60 induced to differentiate in monocyte and human embryonic kidney (HEK) 293 T cells expressing CD14 can produce presepsin by the stimulation of pathogen - associated molecular patterns (PAMPs). We observed presepsin production in both HL-60 and HEK 293T cells, not only in the presence of E. coli but also in its absence. We also observed that E. coli stimulation enhanced the presepsin production in HL-60, but did not enhance it in HEK293T cells. These results suggest that there is a presepsin production pathway requiring no stimulation of PAMPs such as E. coli in a healthy person, and that this pathway is different from presepsin production in immune cells during sepsis."}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"プレセプシンは敗血症のバイオマーカーであり、単球膜上のCD14が貪食時に分解され産生される。しかし、プレセプシンは健常人にも存在しており、その産生機序についての報告はない。本研究では健常人からのプレセプシン産生機序を探索するため、単球系に分化させたHL-60および、CD14を発現させた293T細胞における病原体関連分子パターン(PAMPs)刺激によるプレセプシン産生を検討した。HL-60、293T細胞ともに、Escherichia coli (E. coli) 刺激がない状態においてもプレセプシン産生が認められた。しかしE. coli刺激により産生が増強されたのはHL-60のみであった。これらの結果から健常人の体内においてもE. coliなどのPAMPs刺激を必要としないプレセプシン産生経路が存在し、敗血症時に免疫細胞内で起こる経路とは異なることが示唆された。"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.20608/00000963","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"神戸常盤大学・神戸常盤大学短期大学部"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18845487","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"溝越, 祐志"},{"creatorName":"ミゾコシ, ユウジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"澤村, 暢"},{"creatorName":"サワムラ, トオル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"鈴木, 高史"},{"creatorName":"スズキ, タカシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"MIZOKOSHI, Yuji","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAWAMURA, Toru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUZUKI, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-04-21"}],"displaytype":"detail","filename":"Bulletin11_12.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Bulletin11_12.pdf","url":"https://kobe-tokiwa.repo.nii.ac.jp/record/973/files/Bulletin11_12.pdf"},"version_id":"25982e25-d904-4b37-ad54-edfba6db3392"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"敗血症"},{"subitem_subject":"プレセプシン"},{"subitem_subject":"貪食"},{"subitem_subject":"CD14"},{"subitem_subject":"sepsis"},{"subitem_subject":"presepsin"},{"subitem_subject":"phagocytosis"},{"subitem_subject":"CD14"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"貪食を介さないプレセプシン産生機序に関する検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"貪食を介さないプレセプシン産生機序に関する検討"},{"subitem_title":"Mechanism of presepsin production through a phagocytosis -independent pathway","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"3","path":["71"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-03-31"},"publish_date":"2018-03-31","publish_status":"0","recid":"973","relation_version_is_last":true,"title":["貪食を介さないプレセプシン産生機序に関する検討"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T13:16:42.519436+00:00"}