| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2017-07-26 |
| タイトル |
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|
タイトル |
マウス血清の補体活性測定法の確立－ザイモザン活性化によるreactive lysisを利用した方法の検討－ |
| タイトル |
|
|
タイトル |
New Hemolytic Assay for Mouse Serum Complement Activity - application of zymosan-mediated reactive lysis - |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
主題 |
マウス補体価測定 |
| キーワード |
|
|
主題 |
reactive lysis |
| キーワード |
|
|
主題 |
ザイモザン |
| キーワード |
|
|
主題 |
CH50 |
| キーワード |
|
|
主題 |
ACH50 |
| 資源タイプ |
|
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資源タイプ |
departmental bulletin paper |
| 著者 |
北野, 悦子
畑中, 道代
北村, 肇
KITANO, Etsuko
HATANAKA, Michiyo
Kitamura, Hajime
|
| 抄録（英） |
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内容記述 |
Serum complement activities of human and several experimental animals are usually estimated by the hemolytic assay using sensitized sheep erythrocytes and/or unsensitized rabbit erythrocytes. However, it is well known that these erythrocytes are hardly lysed by incubation with mouse serum and that mouse serum complement activity cannot be measured easily by hemolytic or any other assay. The present study deals with a new hemolytic assay for complement activities of mouse serum, in which the activity is estimated from the degree of hemolysis of unsensitized sheep erythrocytes after incubation of serum in the presence of zymosan. It was confirmed that serum complement activation is responsible for the hemolysis, since the hemolysis was not observed when erythrocytes were incubated i） in buffer containing EDTA, ii） by heatinactivated serum, iii） by C5-deficient mouse serum or iv） in the absence of zymosan. The mechanism of hemolysis is so-called the reactive lysis（ deviated lysis, bystander lysis）, in which erythrocytes are lysed when serum complement activation proceeds not on the erythrocyte membrane but in the fluid phase close to the erythrocytes. Since this assay is simply and easily handled, and does not require any special reagent or treatment of serum and cells, the method may contribute widely to experimental biology. |
| 書誌情報 |
神戸常盤大学紀要
en : Bulletin of Kobe Tokiwa University
号 1,
p. 1-8,
発行日 2009-11-30
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| 出版者 |
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出版者 |
神戸常盤大学・神戸常盤大学短期大学部 |
| ISSN |
|
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
21884781 |
| 論文ID（NAID） |
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|
識別子タイプ |
NAID |
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関連識別子 |
40016961744 |
Bulletin01_01.pdf (995.9 kB)
